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Related Concept Videos

Isolation of Primary Murine Brain Microvascular Endothelial Cells08:14

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Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.
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Related Experiment Video

Updated: Jan 20, 2026

Isolation of Primary Murine Brain Microvascular Endothelial Cells
08:14

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Published on: November 14, 2014

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Microvascular endothelial function and primary open angle glaucoma.

Syed Mudassar Imran Bukhari1, Kiu Kwong Yew1, Rajasunthari Thambiraja1

  • 1Department of Ophthalmology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kota Bharu, Kelantan, Malaysia.

Therapeutic Advances in Ophthalmology
|September 7, 2019
PubMed
Summary
This summary is machine-generated.

Microvascular endothelial dysfunction, characterized by impaired vasodilatation, is a potential risk factor for primary open-angle glaucoma (POAG). This study found significant differences in endothelial function between POAG patients and controls.

Keywords:
glaucomaiontophoresislaser Doppler fluximetrymicrovascular endothelial dysfunction

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Last Updated: Jan 20, 2026

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Area of Science:

  • Ophthalmology
  • Cardiovascular Research
  • Vascular Biology

Background:

  • Primary open-angle glaucoma (POAG) is a leading cause of irreversible blindness globally.
  • The pathophysiology of POAG is complex and multifactorial, involving optic nerve damage.
  • Microvascular endothelial dysfunction is increasingly recognized as a systemic vascular risk factor.

Purpose of the Study:

  • To investigate the association between microvascular endothelial function and POAG.
  • To determine if impaired endothelial function serves as a risk factor for developing POAG.

Main Methods:

  • A cross-sectional study involving 114 Malay patients diagnosed with POAG and 101 control subjects.
  • Microvascular endothelial function assessed using laser Doppler fluximetry and iontophoresis.
  • Endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilatation responses were measured.

Main Results:

  • POAG patients exhibited significantly lower endothelium-dependent (ACh%) and independent (SNP%) vasodilatation compared to controls.
  • These differences persisted after adjusting for potential confounding factors.
  • Age and diastolic blood pressure were inversely correlated with microvascular endothelial function.

Conclusions:

  • Impaired microvascular endothelial function and vasodilatation are present in patients with POAG.
  • Microvascular endothelial function represents a potential risk factor for the development of POAG.