Extracellular Vesicles: From Biology to Biomedical Applications

Esther Serrano-Pertierra1, Maria Carmen Blanco-López2

  • 1Department of Physical and Analytical Chemistry, University of Oviedo, 33006 Oviedo, Spain. serranoesther@uniovi.es.

Summary

The legend of Philippides running from Marathon to Athens highlights ancient communication methods. This historical account underscores the importance of rapid information dissemination in military contexts.

Related Concept Videos

Characterizing Extracellular Vesicles from Biological Fluids05:07

Characterizing Extracellular Vesicles from Biological Fluids

Here, we demonstrate the characterization method for extracellular vesicles (EVs) collected from biological fluids, such as tears and saliva, of human subjects. The scanner used in this method is capable of detecting the phenotype, size, and total particle count of EVs from 1 µL of the...
823
Setting a Successful Sorting for Extracellular Vesicle Isolation08:37

Setting a Successful Sorting for Extracellular Vesicle Isolation

This protocol provides a detailed description of sorting extracellular vesicles (EVs) released by mesenchymal stromal cells. In particular, it focuses on the instrument setting and the optimization of the sorting conditions. The goal is to sort extracellular vesicles while preserving their...
1.6K
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this...
2.5K
Isolation of Tissue Extracellular Vesicles from the Liver05:39

Isolation of Tissue Extracellular Vesicles from the Liver

This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue...
12.4K
Extraction of Extracellular Vesicles from Whole Tissue09:03

Extraction of Extracellular Vesicles from Whole Tissue

Here, we provide a detailed protocol to isolate small extracellular vesicles (EVs) from whole tissues, including brain and tumor specimens. This method offers a reproducible technique to extract EVs from solid tissue for further downstream...
16.0K
Evaluation of the Storage Stability of Extracellular Vesicles11:31

Evaluation of the Storage Stability of Extracellular Vesicles

Here we present a readily applicable protocol to assess the storage stability of extracellular vesicles, a group of naturally occurring nanoparticles produced by cells. The vesicles are loaded with glucuronidase as a model enzyme and stored under different conditions. After storage, their physicochemical parameters and the activity of the encapsulated enzyme are...
15.0K