Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

29.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
29.8K
Structural Protein Function01:56

Structural Protein Function

3.2K
3.2K
Communication01:03

Communication

8.7K
Communication between two animals occurs when one animal transmits an information signal that causes a change in the animal that receives the information. Organisms communicate with one another in a host of different ways. Signals can be auditory, chemical, visual, tactile, or a combination of these. Communication is a critical behavioral adaptation that promotes survival, growth, and reproduction.
8.7K
Communication01:28

Communication

9.5K
Sharing information, concepts, and emotions to foster mutual understanding is communication. The sender, recipient, and transaction must be considered in this manner. The sender is the person who shares the message, the recipient is the person who receives and understands the message, and the transaction is the method used to deliver the message and the variables that affect the communication's context and surroundings. The nurse-client connection is built on therapeutic communication.
9.5K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

1.1K
Computational methods hold promises for expediting drug discovery, yet they frequently overlook the dynamic nature of protein structures. Here, we discuss ensemble-based docking analysis to indirectly incorporate protein flexibility, potentially improving the accuracy and reliability of drug discovery...
1.1K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.0K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Person-Centered Exploration of Neonatal Intensive Care Unit Stressors and Social Support in Parenting Very Preterm Infants: A Cross-Sectional Study on Risks and Resources in Italy and Portugal.

Children (Basel, Switzerland)·2026
Same author

Cytomegalovirus in pregnancy: 20-year experience of a southern italy centre on diagnosis, prenatal surveillance, and neonatal outcomes.

European journal of obstetrics, gynecology, and reproductive biology·2026
Same author

Exploring channelrhodopsin-2 and gold nanocluster interaction: a route to control the protein photocycle.

Nanoscale·2026
Same author

Ultrasound-Guided Non-Tunneled Centrally Inserted Central Catheters versus Peripherally Inserted Central Catheters in Neonates Under 1000 grams: A Comparative Cohort Study.

Neonatology·2026
Same author

Proximity proteomics reveals a co-evolved LRRK2-regulatory network linked to centrosomes.

EMBO reports·2026
Same author

Transient Aggregation-Prone States in Disordered Proteins as Therapeutic Targets: The Amyloid-β Case.

Journal of chemical information and modeling·2026

Related Experiment Video

Updated: Jan 20, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

Interconnecting Flexibility, Structural Communication, and Function in RhoGEF Oncoproteins.

Angelo Felline1, Luca Belmonte1, Francesco Raimondi1

  • 1Department of Life Sciences , University of Modena and Reggio Emilia , via Campi 103 , 41125 Modena , Italy.

Journal of Chemical Information and Modeling
|September 7, 2019
PubMed
Summary
This summary is machine-generated.

Structural differences in Lbc RhoGEF explain its cancer-driving activity. Its flexibility and less stable pathways, unlike its homolog, determine constitutive activity, offering potential drug targets.

More Related Videos

Single-cell Microinjection for Cell Communication Analysis
09:59

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

11.8K
Structural Protein Function: Collagen, Actin, Myosin and Keratin
01:56

Structural Protein Function: Collagen, Actin, Myosin and Keratin

29.8K

Related Experiment Videos

Last Updated: Jan 20, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K
Single-cell Microinjection for Cell Communication Analysis
09:59

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

11.8K
Structural Protein Function: Collagen, Actin, Myosin and Keratin
01:56

Structural Protein Function: Collagen, Actin, Myosin and Keratin

29.8K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Structural Biology

Background:

  • Dbl family Rho guanine nucleotide exchange factors (RhoGEFs) are crucial regulators of RhoA signaling.
  • Aberrant RhoA activity, often driven by oncogenic RhoGEFs like Lbc RhoGEF, contributes to cancer development.

Purpose of the Study:

  • To elucidate the structural and dynamic determinants of the constitutive activity in Lbc RhoGEF compared to its homolog, leukemia-associated RhoGEF.
  • To understand the molecular mechanisms underlying RhoA nucleotide exchange and its regulation by RhoGEFs.

Main Methods:

  • Comparative analysis of protein structures and dynamics in different functional states.
  • Structure network-based analysis to identify allosteric communication pathways.
  • Investigation of RhoA-RhoGEF interactions and their impact on catalytic activity.

Main Results:

  • Lbc RhoGEF exhibits higher intrinsic flexibility and less stable allosteric communication than its homolog, accounting for its constitutive activity.
  • The Dbl homology (DH) and pleckstrin homology (PH) domains are critical for RhoGEF dynamics, which are modulated by RhoA binding.
  • RhoA binding stabilizes allosteric networks within RhoGEF, suggesting a dual role for RhoA as both substrate and regulator.

Conclusions:

  • The structural and dynamic properties of Lbc RhoGEF underpin its oncogenic activity.
  • Understanding these mechanisms reveals potential therapeutic strategies targeting RhoGEF-RhoA interactions.
  • Structure network analysis is a valuable tool for identifying druggable sites in regulatory proteins.