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An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Drug resistance testing for HIV-1 infected individuals failing antiretroviral therapy (ART) can guide future therapies and improve treatment outcomes. Optimizing individual and population health outcomes in high HIV prevalence but resource-limited settings will ultimately require affordable and accessible drug resistance genotyping and interpretation...
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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Quantifying levels of HIV-1 RNA in plasma and sequencing single HIV-1 genomes from individuals with viral loads below the limit of detection (50-75 copies/ml) is difficult. Here we describe how to extract and quantify plasma viral RNA using a real time PCR assay that reliably measures HIV-1 RNA down to 0.3 copies/ml and how to amplify viral genomes by single genome sequencing, from samples with very low viral...
32.1K
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors05:46

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant...
18.3K
COVID-19 / Coronavirus Outbreak: A Rapidly Deployable Medical Facility Optimized for Epidemics (Smart Pod)01:19

COVID-19 / Coronavirus Outbreak: A Rapidly Deployable Medical Facility Optimized for Epidemics (Smart Pod)

The Centers for Disease Control and Prevention (CDC) recommends that healthcare providers perform screening tests on patients with symptoms of COVID-19 in an isolated room to protect other patients from potential exposure to the virus. 
The Smart Pod, a rapidly deployable medical facility that was designed in the wake of the Ebola virus, can serve as a biocontainment unit for airborne pathogens. This emergency solution has been developed by Baylor College of Medicine as a nimble,...
10.3K
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1...
12.7K
Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

This visualized experiment is a guide for utilizing a fluorescent molecular clone of HIV for live confocal imaging...
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