Related Experiment Video

Updated: Jan 20, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

2.4K

Pyrophosphate ion-responsive alginate hydrogel as an effective fluorescent sensing platform for alkaline phosphatase

Yong Li1, Zhen-Zhong Huang, Yuhao Weng

  • 1Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, P. R. China. hltan@jxnu.edu.cn.

Chemical Communications (Cambridge, England)
|September 7, 2019
PubMed

More Related Videos

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations08:57

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

8.3K
A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

7.6K

Related Concept Videos

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence06:33

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

This article provides a detailed methodology for the measurement of isolevuglandins in tissues by immunofluorescence using alkaline phosphatase-conjugated ScFv D11 antibody. Hypertension models in both mice and humans are used to explain the step-by-step procedures and fundamental principles associated with isolevuglandin measurement in tissue samples.
2.4K
Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations08:57

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Engineering and analysis of load bearing tissues with heterogeneous cell populations are still a challenge. Here, we describe a method for creating bi-layered alginate hydrogel discs as a platform for co-culture of diverse cell populations within one construct.
8.3K
Quantification of Bioactive Type-I Interferons using a Secreted Embryonic Alkaline Phosphatase Reporter Assay03:11

Quantification of Bioactive Type-I Interferons using a Secreted Embryonic Alkaline Phosphatase Reporter Assay

This video demonstrates secreted embryonic alkaline phosphatase assay using HEK-Blue interferon-α/β reporter cells to quantify the type-I interferons (IFNs) released following stimulation of plasmacytoid dendritic cells by HIV-1 Infected CD4+ T...
527
A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

This work reports an innovative silicon-tipped fiber-optic sensing platform (Si-FOSP) for high-resolution and fast-response measurement of a variety of physical parameters, such as temperature, flow, and radiation. Applications of this Si-FOSP span from oceanographic research, mechanical industry, to fusion energy...
7.6K
Preparation of Quality Inositol Pyrophosphates10:34

Preparation of Quality Inositol Pyrophosphates

Inositol pyrophosphates play an important role in human pathologies such cancer, diabetes and obesity; however, the exact mechanism of action is a matter of dispute. The lack of commercially available inositol pyrophosphates renders detailed studies problematic. Here we describe a simple protocol to produce and isolate milligrams of inositol...
13.6K
Preparation of Cross-Linked Sodium Alginate Microspheres with Different Metal Ions Using the Microfluidic Electrospray Technology07:24

Preparation of Cross-Linked Sodium Alginate Microspheres with Different Metal Ions Using the Microfluidic Electrospray Technology

The protocol describes the preparation of sodium alginate microspheres cross-linked with different metal ions using a microfluidic device for drug carrier design. The antimicrobial properties and slow drug release of these microspheres were also...
2.9K