Related Experiment Video
Updated: Jan 20, 2026

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Published on: July 28, 2017
Colour tuning and enhancement of gel-based electrochemiluminescence devices utilising Ru(ii) and Ir(iii) complexes
Lachlan C Soulsby1, Egan H Doeven2, Tien T Pham1
1School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Waurn Ponds, Victoria, 3216, Australia. paul.francis@deakin.edu.au.
Abstract:
Combining luminophores in ratios that compensate for energy transfer provides a readily selectable range of new emission colours for gel-based electrochemiluminescence devices (ECLDs). A novel blue ECLD luminophore is also introduced and shown to enhance the intensity of the conventional green emitter through a mixed annihilation ECL mechanism. Peak-to-peak voltages were minimised using asymmetric potential pulse sequences, which increased the longevity of the ECLD emission.
Related Concept Videos
06:36Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
07:44An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
12:55Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
06:09Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay
05:11High-precision Electromagnetic Flowmeter with Empty Pipe Detection via Complex Programmable Logic Device-based Waveform Recognition
12:03Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

