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Related Experiment Video

Updated: Jan 20, 2026

Permeabilization of Adhered Cells Using an Inert Gas Jet
08:21

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Dextranol: An inert xeroprotectant.

Bryan J Jones1, Advitiya Mahajan1, Alptekin Aksan1

  • 1Biostabilization Laboratory, Department of Mechanical Engineering, University of Minnesota, Minneapolis, Minnesota, United States of America.

Plos One
|September 7, 2019
PubMed
Summary

Dextranol, a modified dextran, prevents damaging reactions with dried proteins during storage. This improved xeroprotectant preserves clinical and biologic samples better than unmodified dextran.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Polysaccharide dextran is a xeroprotectant crucial for preserving dried protein samples.
  • Unmodified dextran can react with proteins during storage, forming damaging conjugates.
  • This reaction compromises the integrity of clinical and biologic samples.

Purpose of the Study:

  • To synthesize and evaluate dextranol, a reduced form of dextran, as an improved xeroprotectant.
  • To assess dextranol's ability to prevent protein-polysaccharide conjugation during storage.
  • To determine the efficacy of dextranol in preserving serum biomarkers.

Main Methods:

  • Dextranol was synthesized by reducing dextran's anomeric carbon.
  • Dried serum and purified protein samples were stored in dextran- or dextranol-based matrices.
  • Protein conjugation and biomarker levels were analyzed using immunoassay and other methods.

Main Results:

  • Dextran reacted with dried proteins, forming high-molecular weight Amadori-product conjugates.
  • Dextranol prevented the formation of these damaging conjugates in both serum and purified proteins.
  • Biomarker levels in serum samples stored in dextranol showed significantly less degradation compared to those stored in dextran.

Conclusions:

  • Dextranol is a superior xeroprotectant compared to dextran, effectively preventing protein-polysaccharide conjugation.
  • The modification of dextran to dextranol significantly enhances the stability and preservation of dried protein samples.
  • Dextranol offers a valuable improvement for storing clinical samples and biologic drug compounds.