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Using Luciferase to Image Bacterial Infections in Mice10:23

Using Luciferase to Image Bacterial Infections in Mice

Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence...
21.0K
Oral Bacterial Infection and Shedding in Drosophila melanogaster09:32

Oral Bacterial Infection and Shedding in Drosophila melanogaster

This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial...
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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes14:05

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host...
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Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients13:50

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.
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Wolbachia Bacterial Infection in Drosophila09:46

Wolbachia Bacterial Infection in Drosophila

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Infecting Flies with Bacterial Suspension Using a Nanoinjector03:37

Infecting Flies with Bacterial Suspension Using a Nanoinjector

This video demonstrates the nano-injection of bacterial suspension into the fly body cavity, resulting in bacterial multiplication and infection. The fly's immune response clears some bacteria, while others escape immune clearance and survive within the host causing an...
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