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Related Concept Videos

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

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Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their...
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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions08:17

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A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell...
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Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells08:42

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The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic...
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Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins07:20

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Here, we present a protocol to genetically modify primary or expanded human natural killer (NK) cells using Cas9 Ribonucleoproteins (Cas9/RNPs). By using this protocol, we generated human NK cells deficient for transforming growth factor–b receptor 2 (TGFBR2) and hypoxanthine phosphoribosyltransferase 1...
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Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)09:02

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

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This protocol describes the development, expansion, and in vivo imaging of NK cells derived from hESCs and...
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Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies11:52

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

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Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of...
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Related Experiment Video

Updated: Jan 20, 2026

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

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The RAC2-PI3K axis regulates human NK cell maturation and function

Giovanna Tabellini1, Manuela Baronio2, Ornella Patrizi1

  • 1Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.

Clinical Immunology (Orlando, Fla.)
|September 7, 2019
PubMed
Summary

No abstract available in PubMed .

Keywords:
CytotoxicityNK cellsPI3KRAC2p110d

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