Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT07:10

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

5.5K
We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and...
5.5K
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

662
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and...
662
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

14.4K
A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular...
14.4K
Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning15:53

Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning

15.5K
The overall goal of this protocol is to accurately align magnetic resonance imaging (MRI) image volumes with histology sections via the creation of customized 3D-printed brain holders and slicer...
15.5K
3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry07:10

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

2.0K
The presented method describes how to identify and solve measurement artifacts related to secondary ion mass spectrometry as well as obtain realistic 3D distributions of impurities/dopants in solid state materials.
2.0K
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis06:42

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

22.2K
In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Principled and Data-efficient Information-theoretic Method for Feature Selection.

IEEE journal of biomedical and health informatics·2026
Same author

Quasi-two-dimensional ferroelectricity with multiple switchable polarization states in N-H coinjected perovskite manganites.

Science advances·2025
Same author

Partial information decomposition for discrete target and continuous source random variables.

Physical review. E·2025
Same author

Quantitative comparison of long-range electric field measurements using off-axis electron holography and 4D-STEM via differential phase contrast.

Ultramicroscopy·2025
Same author

Comparison of automatic and physiologically-based feature selection methods for classifying physiological stress using heart rate and pulse rate variability indices.

Physiological measurement·2024
Same author

Testing dynamic correlations and nonlinearity in bivariate time series through information measures and surrogate data analysis.

Frontiers in network physiology·2024

Related Experiment Video

Updated: Jan 20, 2026

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
07:10

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

5.5K

Analysis of depth-sectioning STEM for thick samples and 3D imaging.

Eric G T Bosch1, Ivan Lazić1

  • 1Thermo Fisher Scientific, Achtseweg, Noord 5, 5651GG Eindhoven, NOORD-BRABANT, the Netherlands.

Ultramicroscopy
|September 7, 2019
PubMed
Summary

A new model for 3D volume imaging in scanning transmission electron microscopy (STEM) applies to all techniques. This model analyzes 3D resolution and contrast transfer function for depth-sectioning STEM imaging.

Keywords:
3D imagingDepth sectioning STEMSTEMThick samplesiDPC-STEM

More Related Videos

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

662
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

14.4K

Related Experiment Videos

Last Updated: Jan 20, 2026

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
07:10

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

5.5K
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

662
Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

14.4K

Area of Science:

  • Materials Science
  • Physics
  • Electron Microscopy

Background:

  • Scanning Transmission Electron Microscopy (STEM) is crucial for materials characterization.
  • Depth-sectioning techniques in STEM enable 3D imaging.
  • Existing models often have limitations for various STEM modes.

Purpose of the Study:

  • To derive a universal model for 3D volume imaging in depth-sectioning STEM.
  • To generalize the concept of the undisturbed probe for all STEM techniques.
  • To enable analysis of 3D resolution and contrast transfer function (CTF).

Main Methods:

  • Derivation of a 3D imaging model based on linearity, undisturbed probe, and elastic scattering.
  • Generalization of the point spread function for various STEM modes (ADF-STEM, ABF-STEM, iDPC-STEM).
  • Multi-slice simulations to validate the model for amorphous and crystalline samples.

Main Results:

  • The derived model is valid for all depth-sectioning STEM techniques under specific conditions.
  • Closed expressions for 3D resolution and CTF are obtained, applicable to thick samples.
  • iDPC-STEM shows potential for deeper information extraction and thickness measurement compared to ADF-STEM.

Conclusions:

  • The new model provides a unified framework for understanding 3D imaging in depth-sectioning STEM.
  • Model limitations are identified, particularly for crystalline samples due to channeling.
  • Practical criteria for distinguishing thin and thick samples in STEM are proposed.