Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Quantitative Autonomic Testing11:40

Quantitative Autonomic Testing

58.5K
Standardized, comprehensive and fully quantitative testing of autonomic functions is described. The autonomic tests consist of evaluation of all three major autonomic domains including cardiovagal, adrenergic and sudomotor. The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores.
58.5K
Functions of Connective Tissues01:17

Functions of Connective Tissues

14.8K
Connective tissues perform a broad range of functions in the body. Their primary function is to connect and link different tissues in the body and act as packaging material between tissues. The areolar tissue, a connective tissue prototype, commonly cements various tissue types in diverse body organs. In contrast, adipose tissue cushions internal organs while insulating the body from heat loss.
Hard connective tissues, such as bones and cartilage, provide structure and support to the body.
14.8K
Clinical Application of Phase Angle and BIVA Z-Score Analyses in Patients Admitted to an Emergency Department with Acute Heart Failure04:05

Clinical Application of Phase Angle and BIVA Z-Score Analyses in Patients Admitted to an Emergency Department with Acute Heart Failure

2.8K
In this protocol, we explain how to obtain and interpret phase angle values and bioelectrical impedance vectorial analysis (BIVA) Z-score obtained by bioelectrical impedance in patients with acute heart failure admitted to the Emergency Department and their clinical applicability as a predictive marker for the prognosis of a 90-day...
2.8K
Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice09:40

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

11.1K
Here, we provide a useful approach for studying the mechanism of right ventricular failure. A more convenient and efficient approach to pulmonary artery constriction is established using surgical instruments made inhouse. In addition, methods to evaluate the quality of this approach by echocardiography and catheterization are...
11.1K
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation11:10

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

2.6K
Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit...
2.6K
Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients13:50

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

12.7K
Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.
12.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Layer-specific choroidal vascular adaptation to high-altitude hypoxia revealed by functional OCT at 3,800 m.

Function (Oxford, England)·2026
Same author

Is there a relationship between hypocapnia and tachycardia in postural orthostatic tachycardia syndrome (POTS)? A systematic review.

Clinical autonomic research : official journal of the Clinical Autonomic Research Society·2023
Same author

Thinking from the heart: arrhythmia-induced reduction in cerebral blood flow and the cumulative burden on cognitive function in cardiovascular disease.

The Journal of physiology·2022
Same author

Functional optical coherence tomography at altitude: retinal microvascular perfusion and retinal thickness at 3,800 meters.

Journal of applied physiology (Bethesda, Md. : 1985)·2022
Same author

The molecular makeup of peripheral and central baroreceptors: stretching a role for Transient Receptor Potential (TRP), Epithelial Sodium Channel (ENaC), Acid Sensing Ion Channel (ASIC), and Piezo channels.

Cardiovascular research·2021
Same author

Syncope and silent hypoxemia in COVID-19: Implications for the autonomic field.

Autonomic neuroscience : basic & clinical·2021

Related Experiment Video

Updated: Jan 20, 2026

Quantitative Autonomic Testing
11:40

Quantitative Autonomic Testing

Published on: July 19, 2011

58.5K

Reduced brainstem functional connectivity in patients with peripheral autonomic failure.

Jacquie Baker1, Kurt Kimpinski2

  • 1School of Kinesiology, Western University, London, Ontario, Canada; Department of Clinical Neurological Sciences, University Hospital, London Health Sciences Centre, London, Ontario, Canada.

Neuroimage. Clinical
|September 8, 2019
PubMed
Summary

Patients with autonomic failure exhibit reduced brainstem connectivity to key brain regions involved in autonomic control. This impaired functional connectivity is linked to the severity of their autonomic dysfunction.

Keywords:
Autonomic failureBrainstemCentral autonomic networkFunctional connectivityNeurogenic orthostatic hypotension

More Related Videos

Author Spotlight: Unveiling Prognostic Indicators in Heart Failure - The Role of Phase Angle and Bioelectrical Impedance Analysis
04:05

Author Spotlight: Unveiling Prognostic Indicators in Heart Failure - The Role of Phase Angle and Bioelectrical Impedance Analysis

Published on: June 30, 2023

2.8K
Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice
09:40

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

Published on: May 13, 2019

11.1K

Related Experiment Videos

Last Updated: Jan 20, 2026

Quantitative Autonomic Testing
11:40

Quantitative Autonomic Testing

Published on: July 19, 2011

58.5K
Author Spotlight: Unveiling Prognostic Indicators in Heart Failure - The Role of Phase Angle and Bioelectrical Impedance Analysis
04:05

Author Spotlight: Unveiling Prognostic Indicators in Heart Failure - The Role of Phase Angle and Bioelectrical Impedance Analysis

Published on: June 30, 2023

2.8K
Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice
09:40

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

Published on: May 13, 2019

11.1K

Area of Science:

  • Neuroscience
  • Autonomic Nervous System Research
  • Functional Neuroimaging

Background:

  • Autonomic homeostasis relies on a central autonomic network involving brainstem, cortical, and subcortical structures.
  • Neurogenic orthostatic hypotension (NOH) signifies autonomic failure due to inadequate sympathetic response to postural changes.

Purpose of the Study:

  • To investigate brainstem functional connectivity in patients with NOH and peripheral autonomic lesions.
  • To compare brainstem connectivity between NOH patients and healthy controls during rest, Valsalva maneuver, and recovery.

Main Methods:

  • Functional brain scans (fMRI) were performed on 15 NOH patients and 15 controls.
  • Participants underwent resting-state scans and completed three Valsalva maneuvers.
  • Brainstem functional connectivity to cortical and subcortical regions was analyzed and contrasted between groups.

Main Results:

  • Controls showed significantly greater resting-state brainstem connectivity to the anterior cingulate cortex, insula, putamen, and thalamus compared to NOH patients.
  • During the Valsalva maneuver and recovery, controls exhibited enhanced brainstem connectivity with cerebellar regions, putamen, posterior cingulate cortex, amygdala, and medial prefrontal cortex.
  • A negative correlation was observed between brainstem-cerebellar connectivity and the severity of autonomic dysfunction in NOH patients.

Conclusions:

  • Patients with autonomic failure demonstrate reduced functional coupling between the brainstem and central autonomic network components, including the cerebellum, insula, thalamus, and cingulate cortices.
  • The observed connectivity deficits are associated with the degree of autonomic impairment.
  • These findings suggest impaired brainstem connectivity as a potential mechanism underlying autonomic failure in NOH.