Antimicrobial Synergistic Effect Between Ag and Zn in Ag-ZnO·mSiO₂ Silicate Composite with High Specific Surface Area

Antimicrobial materials are widely used for inhibition of microorganisms in the environment. It has been established that bacterial growth can be restrained by silver nanoparticles. Combining these with other antimicrobial agents, such as ZnO, may increase the antimicrobial activity and the use of carrier substrate makes the material easier to handle. In the paper, we present an antimicrobial nanocomposite based on silver nanoparticles nucleated in general silicate nanostructure ZnO·mSiO₂. First, we prepared the silicate fine net nanostructure ZnO·mSiO₂ with zinc content up to 30 wt% by precipitation of sodium water glass in zinc acetate solution. Silver nanoparticles were then formed within the material by photoreduction of AgNO₃ on photoactive ZnO. This resulted into an Ag-ZnO·mSiO₂ composite with silica gel-like morphology and the specific surface area of 250 m²/g. The composite, alongside with pure AgNO₃ and clear ZnO·mSiO₂, were successfully tested for antimicrobial activity on both gram-positive and gram-negative bacterial strains and yeast Candida albicans. With respect to the silver content, the minimal inhibition concentration of Ag-ZnO·mSiO₂ was worse than AgNO₃ only for gram-negative strains. Moreover, we found a positive synergistic antimicrobial effect between Ag and Zn agents. These properties create an efficient and easily applicable antimicrobial material in the form of powder.