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Determination of phosphate ions with an enzyme sensor system
1Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Biosensors
|January 1, 1987
Summary
A novel enzyme sensor system accurately determines phosphate ions in food. This rapid, reusable sensor offers a quick and efficient method for food analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biosensors
Background:
- Phosphate ions are crucial in food products, necessitating accurate quantification.
- Existing methods for phosphate determination can be time-consuming or complex.
Purpose of the Study:
- To develop a rapid and reliable enzyme sensor for determining phosphate ions.
- To evaluate the performance and reusability of the developed sensor system.
Main Methods:
- Immobilized enzymes and an oxygen electrode were utilized to construct the enzyme sensor.
- The sensor's principle is based on the nucleoside phosphorylase catalyzed reaction, requiring inorganic phosphorus.
Main Results:
- The enzyme sensor system achieved a determination time of within 3 minutes per assay.
- The sensor demonstrated robustness, withstanding at least 70 assays.
- The system proved effective for simple, rapid, and continuous phosphate ion determination in food samples.
Conclusions:
- The developed enzyme sensor offers a highly efficient method for phosphate ion analysis in food.
- This biosensor technology provides a valuable tool for food quality control and safety.