Novel polymerase spiral reaction assay for the visible molecular detection of porcine circovirus type 3

Related Experiment Video

Updated: Jan 20, 2026

Detecting Environmental Microorganisms Using PCR
13:34

Detecting Environmental Microorganisms Using PCR

Published on: April 30, 2023

47.0K

Jun Ji1, Xin Xu2, Xueyu Wang2

  • 1Henan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, 1638 Wolong Road, Nanyang, Hena, 473061, People's Republic of China. jijun020@126.com.

BMC Veterinary Research
|September 8, 2019
PubMed
Abstract

Keywords:
Molecular detectionPCV3Polymerase spiral reactionVisible detection

More Related Videos

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses08:10

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Published on: May 2, 2019

8.8K
PCR: Principle, Instrumentation, and Applications13:27

PCR: Principle, Instrumentation, and Applications

Published on: April 30, 2023

763.3K

Related Concept Videos

Detecting Environmental Microorganisms with the Polymerase Chain Reaction and Gel Electrophoresis13:34

Detecting Environmental Microorganisms with the Polymerase Chain Reaction and Gel Electrophoresis

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Bradley Schmitz
Polymerase chain reaction (PCR) is a technique used to detect microorganisms that are present in soil, water, and atmospheric environments. By amplifying specific sections of DNA, PCR can facilitate the detection and identification of target microorganisms down to the species, strain, and serovar/pathovar level. The technique can also be utilized to characterize entire...
47.0K
PCR: The Polymerase Chain Reaction13:27

PCR: The Polymerase Chain Reaction

The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling – cyles of temperature changes at fixed time intervals. Using a thermostable DNA polymerase, PCR can create numerous copies of DNA from DNA building blocks called dNTPs. There are three steps in PCR: denaturation, annealing, and elongation. Denaturation is the first step in the cycle and causes the DNA to melt by disrupting hydrogen bonds between the bases resulting in single-stranded DNA.
763.3K
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses08:10

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies...
8.8K
Digital Droplet Polymerase Chain Reaction for Indel Mutation Detection in Target Genes04:34

Digital Droplet Polymerase Chain Reaction for Indel Mutation Detection in Target Genes

This video demonstrates the role of digital droplet polymerase chain reaction in identifying indel mutation in a DNA sample. The high precision and sensitivity of ddPCR enable accurate and consistent detection of rare mutations present in a sample, even at low levels. It can identify and quantify indel mutations in different regions of the genome, providing valuable insights into the mechanisms of mutation and the functional consequences of these...
1.7K
Multiplex Droplet Polymerase Chain Reaction to Detect DNA Methylation in Leukocytes04:28

Multiplex Droplet Polymerase Chain Reaction to Detect DNA Methylation in Leukocytes

This video demonstrates the multiplex droplet polymerase chain reaction technique for the simultaneous quantification of methylated DNA regions in different white blood cell populations as an early biomarker for clinical diagnosis and...
612
Droplet Digital Polymerase Chain Reaction: A Method to Generate Nanodroplet PCR Reactions for Detecting Rare Tumor Mutations04:07

Droplet Digital Polymerase Chain Reaction: A Method to Generate Nanodroplet PCR Reactions for Detecting Rare Tumor Mutations

In this video, we present a step-by-step procedure to generate emulsified nano-droplets to detect target nucleic acids by digital polymerase chain reaction or...
2.8K