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Related Experiment Videos

Disinfectant testing using a modified use-dilution method: collaborative study.

E C Cole1, W A Rutala, G P Samsa

  • 1University of North Carolina School of Medicine, Department of Medicine, Chapel Hill 27599.

Journal - Association of Official Analytical Chemists
|November 1, 1988
PubMed
Summary
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The AOAC use-dilution method (UDM) for disinfectant testing showed significant variability among laboratories even after modifications. This interlaboratory variability questions the UDM's reliability for disinfectant registration purposes.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • The AOAC use-dilution method (UDM) is a standard for testing bactericidal disinfectant efficacy.
  • An initial collaborative study revealed extreme variability in UDM results across multiple laboratories.
  • This variability raised concerns about the method's reliability for disinfectant registration.

Purpose of the Study:

  • To assess if modifications to the UDM could reduce interlaboratory variability in disinfectant efficacy testing.
  • To determine if the revised UDM is suitable for regulatory purposes.

Main Methods:

  • A second collaborative trial involved 12 laboratories testing 6 disinfectants against 3 standard organisms (Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa).
  • Laboratories processed 60 replicates per disinfectant and organism using the modified UDM.

Related Experiment Videos

  • Variance components analysis was used to quantify interlaboratory variability.

    • Main Results:

    • Significant interlaboratory variability persisted for all tested organisms, including Pseudomonas aeruginosa.
    • The modified UDM did not significantly reduce the variance attributed to different laboratories.
    • Results showed a wide range of positive penicylinders across laboratories for the tested disinfectants.

    Conclusions:

    • The modifications made to the UDM did not adequately reduce interlaboratory variability.
    • The continued variability questions the suitability of both the original and modified UDM for disinfectant registration.
    • Further improvements are needed to ensure consistent and reliable disinfectant efficacy testing.