Related Experiment Video

Updated: Jan 19, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

26.7K

A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells

Haoran Guo1,2, Yan Li2, Guanchen Liu2

  • 1Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Institute of Translational Medicine, First Hospital, Jilin University, Changchun, Jilin, 130021, China.

Nature Communications
|September 8, 2019
PubMed
Summary

A second viral gene, ORF2p, is crucial for human enterovirus intestinal infections. Its expression aids virus replication and release from intestinal cells, impacting viral spread.

More Related Videos

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages09:39

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Published on: May 30, 2013

10.5K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.1K

Related Concept Videos

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in...
26.7K
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages09:39

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival...
10.5K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Translating ribosomes decode three nucleotides per codon into peptides. Their movement along mRNA, captured by ribosome profiling, produces the footprints exhibiting characteristic triplet periodicity. This protocol describes how to use RiboCode to decipher this prominent feature from ribosome profiling data to identify actively translated open reading frames at the whole-transcriptome...
4.1K
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids08:04

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

This protocol describes the measurement of epithelial barrier permeability in real-time following pharmacologic treatment in human intestinal organoids using fluorescent microscopy and live cell...
14.8K
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.
9.1K
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells08:22

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

This protocol offers detailed instructions for establishing murine small intestine organoids, isolating type-1 innate lymphoid cells from the murine small intestine lamina propria, and establishing 3-dimensional (3D) co-cultures between both cell types to study bi-directional interactions between intestinal epithelial cells and type-1 innate lymphoid...
6.5K