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Updated: Jan 19, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Josh Tycko1, Michael Wainberg2, Georgi K Marinov1

  • 1Department of Genetics, Stanford University, Stanford, CA, 94305, USA.

Nature Communications
|September 8, 2019
PubMed
Summary

Pooled CRISPR screens can identify essential regulatory elements, but off-target effects are a concern. GuideScan specificity scores effectively filter confounding guide RNAs, enabling accurate identification of regulatory elements.

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