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Updated: Jan 19, 2026

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
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Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors.

Yujiao Zhu1,2, Ziyu Huang3, Qingming Chen1

  • 1Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong, China.

Nature Communications
|September 8, 2019
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Summary

Immobilizing D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in microfluidic reactors boosts enzyme stability and reusability for continuous glucose precursor synthesis, potentially aiding food production.

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Biocatalysis

Background:

  • Natural photosynthesis is limited by the low efficiency of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO).
  • Improving RuBisCO activity and specificity is crucial for enhancing food production in crops.
  • Artificial photosynthesis offers a potential solution to overcome natural limitations.

Purpose of the Study:

  • To develop a method for enhancing RuBisCO stability and reusability.
  • To demonstrate continuous synthesis of glucose precursors using immobilized RuBisCO.
  • To explore the potential of artificial photosynthesis for food production and space colonization.

Main Methods:

  • Immobilization of RuBisCO into a microfluidic reactor.
  • Continuous synthesis of glucose precursor from CO2 and ribulose-1,5-bisphosphate.
  • Assessing enzyme stability (storage and thermal) and reusability through multiple cycles.

Main Results:

  • Continuous glucose precursor production achieved at 13.8 μmol g-1 RuBisCO min-1.
  • RuBisCO immobilization significantly enhanced storage stability (7.2-fold) and thermal stability (6.7-fold).
  • Immobilized RuBisCO retained 90.4% activity after 5 cycles and 78.5% after 10 cycles.

Conclusions:

  • Immobilized RuBisCO in microfluidic reactors offers a stable and reusable biocatalyst for glucose precursor synthesis.
  • This approach mimics natural photosynthesis's light-independent reactions, paving the way for artificial food material production.
  • The technology shows promise for addressing food crises and supporting future space exploration.