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Surface markers of porcine lymphocytes and distribution in various lymphoid organs
Three rosette test systems were developed for the classification of subpopulations of porcine lymphocytes and correlated with the presence or the absence of membrane immunoglobulins, as well as agg-P-IgG receptors, by means of various RFC depletion experiments. Among the ED-RFC negative cells, PBL with surface immunoglobulins (SIg), at 4 degrees C, can be subdivided into two about equal populations: (1) One half of these cells have surface stable SIg (B cells) and mu determinants; they possess C3b receptors leading to the formation of rosettes with complement-coated zymosan particles (ZC). (2) The other half have surface labile Ig when the cells are washed at 37 degrees C (L-cells); they possess Fc receptors as detected by EA-RFC, i. e. pig-IgG-coated ox RBC. Receptors for Fc-agg, as detected by agg-P-IgG, were primarily shown on B cells. ZC and EA-RFC are in the highest tissue concentration, in bone marrow and spleen, respectively, and more numerous in adults than in newborns. Moreover, the number of EA-RFC was twofold higher than that of ZC-RFC in spleen, mesenteric lymph node and cisterna chyli. This latter organ was the only one without null cell.
Three rosette test systems were developed for the classification of subpopulations of porcine lymphocytes and correlated with the presence or the absence of membrane immunoglobulins, as well as agg-P-IgG receptors, by means of various RFC depletion experiments. Among the ED-RFC negative cells, PBL with surface immunoglobulins (SIg), at 4 degrees C, can be subdivided into two about equal populations: (1) One half of these cells have surface stable SIg (B cells) and mu determinants; they possess C3b receptors leading to the formation of rosettes with complement-coated zymosan particles (ZC). (2) The other half have surface labile Ig when the cells are washed at 37 degrees C (L-cells); they possess Fc receptors as detected by EA-RFC, i. e. pig-IgG-coated ox RBC. Receptors for Fc-agg, as detected by agg-P-IgG, were primarily shown on B cells. ZC and EA-RFC are in the highest tissue concentration, in bone marrow and spleen, respectively, and more numerous in adults than in newborns. Moreover, the number of EA-RFC was twofold higher than that of ZC-RFC in spleen, mesenteric lymph node and cisterna chyli. This latter organ was the only one without null cell.