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First detection of multiresistance pRE25-like elements from Enterococcus spp. in Staphylococcus pseudintermedius

Jung-Hun Kang1, Cheol-Yong Hwang1

  • 1Laboratory of Veterinary Dermatology and The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.

Journal of Global Antimicrobial Resistance
|September 8, 2019
PubMed
Abstract

Insights

Multidrug-resistant Staphylococcus pseudintermedius (MDRSP) in Korea carry pRE25-like elements, previously found in Enterococcus. These elements contribute to antimicrobial resistance in canine pyoderma strains, highlighting the need for surveillance.

Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • Enterococcus pRE25 is a known multiresistance plasmid.
  • pRE25-like elements can transfer vancomycin resistance to Staphylococcus aureus.
  • The role of pRE25-like elements in multidrug resistance in Staphylococcus species was previously uninvestigated.

Purpose of the Study:

  • To investigate the presence and characteristics of pRE25-like elements in multidrug-resistant Staphylococcus pseudintermedius (MDRSP).
  • To determine the contribution of these elements to antimicrobial resistance in canine MDRSP strains.

Main Methods:

  • Isolation and characterization of 46 MDRSP strains from canine pyoderma in Korea.
  • Multilocus sequence typing (MLST) and PCR for pRE25-like elements.
  • Whole-genome sequencing (WGS) of four selected isolates.

Main Results:

  • Chromosomal 22-kb pRE25-like elements in MDRSP contained five antimicrobial resistance genes (cat, erm(B), aphA-3, aadK, sat4) and mobile genetic elements.
  • These elements showed high homology (99.8% identity) to the enterococcal pRE25 plasmid.
  • pRE25-like elements were detected in 52.2% of MDRSP isolates, with diverse sequence types (STs), including 12 novel STs in Korea.

Conclusions:

  • pRE25-like elements are prevalent in MDRSP from companion dogs in Korea.
  • These elements likely contribute to the multidrug resistance phenotype in these staphylococcal strains.
  • Continuous surveillance is recommended for pRE25-like elements in staphylococci from both animals and humans due to close human-animal contact.
Keywords:
Multidrug resistanceStaphylococcus pseudintermediuspRE25-like elements

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