Design and synthesis of fluorogenic substrate-based probes for detecting Cathepsin B activity

Related Experiment Video

Updated: Jan 19, 2026

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels
03:05

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels

2.6K

Shusheng Wang1, Brady G Vigliarolo1, Morshed A Chowdhury1

  • 1Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada.

Bioorganic Chemistry
|September 8, 2019
PubMed

Keywords:
Cathepsin BFluorogenic probeLysosomal trappingProdrug linker

More Related Videos

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism09:36

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism

Published on: July 18, 2018

8.3K
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

10.2K

Related Concept Videos

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels03:05

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels

In this video, we perform zymography, an electrophoretic technique, to analyze the proteolytic activity within biological...
2.6K
Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism09:36

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism

We report a solution-based method to synthesize substrate-bound Au nanowires. By tuning the molecular ligands used during the synthesis, the Au nanowires can be grown from various substrates with different surface properties. Au nanowire-based nanostructures can also be synthesized by adjusting the reaction...
8.3K
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex...
10.2K
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities09:10

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good...
7.1K
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

The present protocol describes a method to detect reactive oxygen species (ROS) in the intestinal murine organoids using qualitative imaging and quantitative cytometry assays. This work can be potentially extended to other fluorescent probes to test the effect of selected compounds on...
4.7K
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

PAD4 is an enzyme responsible for the conversion of peptidyl-arginine to peptidyl-citrulline. Dysregulation of PAD4 has been implicated in a number of human diseases. A facile and high-throughput compatible fluorescence based PAD4 assay is...
10.3K