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Updated: Jan 19, 2026

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Published on: June 9, 2019
Kidney harvesting and metabolite extraction for metabolomics studies in rodents
Igor Maksimovic1, Song Zhang2, Ivan Vuckovic2
1Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, United States; Tri-Institutional PhD Program in Chemical Biology, New York, NY, United States.
Abstract:
Elucidating the metabolic changes that accompany disease states via metabolomics analysis of tissues has become an important avenue of exploration in biomarker and therapeutic target discovery. Conventional harvesting techniques rely on post-euthanasia tissue harvest which introduces ischemic conditions and subsequent metabolome changes that may ultimately introduce artifacts into final analyses. In this chapter, we present protocols for low-ischemia time rapid kidney tissue harvest followed by metabolite extraction for metabolomics studies in rodents.
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