Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model21:16

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

69.0K
In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical...
69.0K
In Vitro Transcription Assays and Their Application in Drug Discovery09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

15.6K
In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial...
15.6K
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration09:24

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

23.8K
This study focuses on an in vitro model of wound healing (scratch assay) as a mechanism for determining how environmental contaminants such as arsenic influence cellular migration. The results demonstrate that this in vitro assay provides rapid and early indications of changes to cellular migration prior to in vivo...
23.8K
Thiobarbituric Acid-Reactive Substances Assay to Assess Oxidative Stress in Human Serum03:35

Thiobarbituric Acid-Reactive Substances Assay to Assess Oxidative Stress in Human Serum

1.2K
This video demonstrates the thiobarbituric acid reactive substances (TBARS) assay to assess oxidative stress in biological...
1.2K
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay06:19

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

31.6K
The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density...
31.6K
Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice11:19

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

10.5K
We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectral characterization of intraoperative renal perfusion using hyperspectral imaging and artificial intelligence.

Scientific reports·2024
Same author

Unraveling the parahormetic mechanism underlying the health-protecting effects of grapeseed procyanidins.

Redox biology·2023
Same author

Salivary proteomic profile of young healthy subjects.

Frontiers in molecular biosciences·2023
Same author

Association between mediterranean diet and metal(loid) exposure in 4-5-year-old children living in Spain.

Environmental research·2023
Same author

Benchmarking outcomes for distal pancreatectomy: critical evaluation of four multicenter studies.

Langenbeck's archives of surgery·2023
Same author

Development of a Defined Approach for Eye hazard identification of chemicals having surfactant properties according to the three UN GHS categories.

Toxicology in vitro : an international journal published in association with BIBRA·2023
Same journal

The NTP Chronic Inhalation Study Does Not Support an Inherent Lung Cancer Hazard of Talc: Implications of Lung Particle Overload and Maximum Tolerated Dose.

Regulatory toxicology and pharmacology : RTP·2026
Same journal

Consideration of Carcinogenicity and Mode of Action Information by an Independent Expert Panel to Support Derivation of No-Significant-Risk-Level Values for Vinyl Acetate Monomer.

Regulatory toxicology and pharmacology : RTP·2026
Same journal

Which carcinogenicity study should I use? Automated identification of reliable studies.

Regulatory toxicology and pharmacology : RTP·2026
Same journal

Adoption of artificial intelligence in drug review across the lifecycle: Transformation of regulatory decision-making.

Regulatory toxicology and pharmacology : RTP·2026
Same journal

Cardiovascular outcomes following intrauterine and lactational exposure to cyantraniliprole in male Wistar rats.

Regulatory toxicology and pharmacology : RTP·2026
Same journal

Pesticide residue and mycotoxin occurrence in apples and their impact on human health in Morocco.

Regulatory toxicology and pharmacology : RTP·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

69.0K

In vitro RHE skin sensitisation assays: Applicability to challenging substances.

A Mehling1, E Adriaens2, S Casati3

  • 1BASF Personal Care and Nutrition GmbH, Duesseldorf, Germany.

Regulatory Toxicology and Pharmacology : RTP
|September 9, 2019
PubMed
Summary
This summary is machine-generated.

New in vitro methods using reconstructed human epidermis (RHE) models show promise for identifying skin sensitisation hazards. These alternative approaches may help avoid animal testing for a diverse range of chemicals.

Keywords:
Hazard identificationIn vitro alternativesReconstructed human epidermisSkin sensitisation

More Related Videos

In Vitro Transcription Assays and Their Application in Drug Discovery
09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

15.6K
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
09:24

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

Published on: February 23, 2019

23.8K

Related Experiment Videos

Last Updated: Jan 19, 2026

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

69.0K
In Vitro Transcription Assays and Their Application in Drug Discovery
09:28

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

15.6K
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
09:24

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

Published on: February 23, 2019

23.8K

Area of Science:

  • Toxicology
  • Dermatology
  • In vitro toxicology

Background:

  • Alternative methods for skin sensitisation hazard identification are crucial for the 3Rs (Replacement, Reduction, Refinement).
  • Previous validation of non-animal methods, like the local lymph node assay, faced challenges with diverse chemical substances.
  • Emerging in vitro alternatives need evaluation for their efficacy with difficult-to-assess chemicals.

Purpose of the Study:

  • To evaluate the performance of emerging in vitro alternatives for skin sensitisation hazard identification.
  • To assess a range of challenging substances using reconstructed human epidermis (RHE) models.
  • To determine the predictive accuracy and sensitivity of these assays.

Main Methods:

  • Twelve substances, including known skin sensitisers, were tested.
  • Four in vitro assays utilizing reconstructed human epidermis (RHE) models were employed.
  • Hazard identification was benchmarked against human data.

Main Results:

  • Three of the four RHE-based assays demonstrated an overall predictive accuracy of approximately 70%.
  • One assay (SensCeeTox) showed predictive accuracy below 50% when compared to human data.
  • High sensitivity was observed across most assays, leading to overprediction of sensitisation in some cases.

Conclusions:

  • 3D reconstructed human epidermis (RHE) models show potential as a valuable tool for assessing skin sensitisation.
  • These in vitro methods offer a viable alternative to animal testing for a range of chemicals.
  • Further refinement may be needed to address overprediction and improve accuracy for specific assays.