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07:53Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
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To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.
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09:28Traction Force Microscopy to Study B Lymphocyte Activation
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Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data...
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08:10Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
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This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force...
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12:26Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same...
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02:44Traction Cytometry Assay: A Method to Quantify Traction Force Exerted by Cancer Cells on ECM
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This video describes the traction force microscopy assay. The assay determines the cellular traction of human colon cancer cells on the extracellular matrix present on soft elastic hydrogel having embedded fluorescent beads. The resultant displacement of fluorescent beads is measured using an appropriate...
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10:53Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
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Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical...
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