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Molecular Entanglement and Electrospinnability of Biopolymers07:59

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Electrospinning is a fascinating technique used to fabricate micro- to nano-scale fibers from a wide variety of materials. Molecular entanglement of the constituent polymers in the spinning dope is essential for successful electrospinning. We present a protocol for utilizing rheology to evaluate the electrospinnability of two biopolymers, starch and...
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Begin with a strain of Pseudomonas aeruginosa bacteria, isolated from oil-contaminated soil, in a medium containing a nitrogen source and an excess of edible oil as the carbon source.This strain synthesizes polyhydroxyalkanoate (PHA), a long-chain polymer useful in producing bioplastics.Incubate under agitation to facilitate aeration and promote bacterial growth.Initially, the medium supplies both carbon and nitrogen sources to support bacterial proliferation and generate high biomass.After...
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Begin with an anesthetized rat bearing a brain tumor composed of proliferating tumor cells.Expose the rat’s skull and create a hole to reveal the brain region with the tumor.Take a thermally responsive biopolymer fused with a cell-penetrating peptide and an inhibitor of c-myc, an oncogenic protein.Inject the biopolymer into the femoral vein of the hind limb for delivery to the brain.Apply infrared light through the skull hole to heat the tumor site, which induces biopolymer aggregation...
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Related Experiment Video

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Polyhydroxyalkanoates and Other Biopolymers

Guo-Qiang Chen1, Ann-Christine Albertsson2

  • 1School of Life Sciences, Center for Synthetic and Systems Biology (CSSB) , Tsinghua University , Beijing 100084 , China.

Biomacromolecules
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PubMed
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No abstract available in PubMed .

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