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Updated: Jan 19, 2026
Loop-Mediated Isothermal Amplification for Bacterial Gene Detection
Published on: November 28, 2025
Visual, sensitive and rapid event-specific detection of genetically modified potato EH92-527-1 by loop-mediated
Yuan-Kai Tu1, Yen-Chun Lin1, Yu-Wei Feng1
1Biotechnology Division, Taiwan Agricultural Research Institute, Taichung City, Taiwan.
Abstract:
To date, studies on the application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified organisms (GMOs) are stably increasing and demonstrates LAMP is a potential and promising method for on spot identification of GMOs. However, little information is known for detection of GM potato events by LAMP. In this report, we developed an optimized and visual LAMP assay with high specificity and sensitivity to rapidly amplify genomic DNA of potato EH92-527-1 within 45 min. The limit of detection of LAMP in our study is 10-fold higher than the conventional PCR. Furthermore, LAMP products can be directly observed via naked eyes by addition of SYBR Green I without gel electrophoresis analysis and PCR-based equipment. Therefore, the LAMP assay developed in this paper provides an efficient, convenient and cost-effective tool for the detection of GM potato EH92-527-1.
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