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Related Concept Videos

The Mitotic Spindle02:27

The Mitotic Spindle

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The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
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Histone Variants at the Centromere02:30

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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic...
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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes10:09

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Error in chromosome segregation is a common feature in oocytes. Therefore, studying the spindle assembly checkpoint gives important clues about the mechanisms needed to produce healthy eggs. The present protocol describes three complementary assays to evaluate spindle assembly checkpoint integrity in mouse...
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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations07:14

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Here we present a protocol to assess the dynamics of spindle formation and mitotic progression. Our application of time-lapse imaging enables the user to identify cells at various stages of mitosis, track and identify mitotic defects, and analyze spindle dynamics and mitotic cell fate upon exposure to anti-mitotic...
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Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

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Related Experiment Video

Updated: Jan 19, 2026

Organization of the Mitotic Spindle
02:27

Organization of the Mitotic Spindle

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Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity.

Simon Gemble1, Anthony Simon2, Carole Pennetier2

  • 1Institut Curie, PSL Research University, CNRS, UMR144, Biology of centrosomes and genetic instability lab, 75005 Paris, France; Institut Curie, PSL Research University, CNRS, UMR144, Molecular Mechanisms of Chromosome Dynamics lab, 75005 Paris, France.

Current Biology : CB
|September 10, 2019
PubMed
Summary

Centromere defects, caused by removing centromere protein A (CENP-A), disrupt mitotic spindle poles. This leads to chromosome mis-segregation, highlighting centromeres

Keywords:
centromerescentrosomeschromosome mis-segregationmicrotubule dynamicsmitotic spindle pole integrity

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Related Experiment Videos

Last Updated: Jan 19, 2026

Organization of the Mitotic Spindle
02:27

Organization of the Mitotic Spindle

7.8K
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

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Histone H3 variant at the Centromere: CENP-A
02:30

Histone H3 variant at the Centromere: CENP-A

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Centromeres, marked by CENP-A, and centrosomes nucleate microtubules for chromosome segregation during mitosis.
  • The constitutive centromere associated network (CCAN) and kinetochores are essential for microtubule attachment.
  • Centrosomes, containing centrioles and pericentriolar material (PCM), are the primary microtubule nucleation sites in animal cells.

Purpose of the Study:

  • To investigate the functional relationship between centromeres and centrosomes during mitosis.
  • To determine if centromere dysfunction impacts mitotic spindle pole integrity and chromosome segregation.

Main Methods:

  • High-resolution microscopy was employed in human cells.
  • Rapid removal of CENP-A was used to perturb centromere function.
  • Analysis of mitotic spindle pole integrity, microtubule dynamics, and chromosome mis-segregation.

Main Results:

  • Perturbation of centromere function led to impaired mitotic spindle pole integrity.
  • Observed defects included release of microtubule minus-ends, PCM dispersion, and centriole mis-positioning.
  • These defects were linked to abnormal microtubule dynamics and faulty kinetochore-microtubule attachments.

Conclusions:

  • Centromere function is unexpectedly crucial for maintaining mitotic spindle pole integrity.
  • Restoring spindle pole integrity after centromere inactivation reduced chromosome mis-segregation.
  • This study reveals a novel link between centromeres and mitotic accuracy, essential for genetic stability.