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Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure08:17

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of...
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Phase-Resolved Functional Lung MRI for Pulmonary Ventilation and Perfusion (V/Q) Assessment05:56

Phase-Resolved Functional Lung MRI for Pulmonary Ventilation and Perfusion (V/Q) Assessment

Here, we describe the implementation of phase-resolved functional lung MRI as a contrast-agent-free proton MR technique for the assessment of pulmonary ventilation and perfusion dynamics. Validated and applicable across different field strengths and age groups, it could enhance clinical decision-making in the future by aiding in disease quantification and therapy...
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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment...
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Whole-Body Nanoparticle Aerosol Inhalation Exposures10:11

Whole-Body Nanoparticle Aerosol Inhalation Exposures

A whole-body nanoparticle aerosol inhalation exposure facility was constructed for nano-sized titanium dioxide (TiO2) inhalation toxicology studies. This system provides nano-TiO2 aerosol test atmospheres that have: 1) a steady mass concentration; 2) a homogenous composition free of contaminants; and 3) a stable particle size distribution during aerosol...
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In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface07:05

In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface

We establish a standardized in vitro model for studying cannabis vapor exposure at the air-liquid interface. This model provides a systematic approach to examine the impacts of vaporized cannabis, addressing the growing interest in cannabis vaporizers as an alternative to...
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Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling05:47

Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling

Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical...
21.9K