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Revival and Growth of Lactic Acid Bacteria
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Simple and Rapid Method for Separating Lactic Acid Bacteria from Commercially Prepared Yogurt.

Hidenobu Nakao1, Je-Deok Kim1

  • 1National Institute for Materials Science (NIMS).

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|September 10, 2019
PubMed
Summary

A new method effectively separates lactic acid bacteria (LAB) from yogurt using vortexing to denature casein. This allows for clear single-cell observation and analysis of LAB, aiding in studies of their enzyme activity and protein expression.

Keywords:
Lactic acid bacteriacasein micellesseparationvortexing

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Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Yogurt contains lactic acid bacteria (LAB), crucial for fermentation and gut health.
  • Current methods for isolating LAB from yogurt can be complex and time-consuming.
  • Accurate analysis of LAB requires pure, intact cell samples.

Purpose of the Study:

  • To develop a simple and efficient method for isolating lactic acid bacteria (LAB) from yogurt.
  • To enable direct single-cell level analysis of LAB morphology and characteristics.
  • To provide a tool for studying LAB enzyme activity and protein expression.

Main Methods:

  • Utilized vortexing to induce destruction and denaturation of casein micelle aggregates in yogurt.
  • Separated the resulting mixture to obtain samples enriched with lactic acid bacteria.
  • Employed microscopy for direct observation and verification of isolated LAB.

Main Results:

  • Successfully isolated lactic acid bacteria (LAB) from yogurt samples.
  • The vortexing method effectively removed casein, yielding LAB-only samples.
  • Microscopy confirmed the clear observability of recovered LAB at the single-cell level.
  • Morphological changes in LAB could be directly detected.

Conclusions:

  • The developed vortexing method offers a simple and effective way to isolate LAB from yogurt.
  • This technique facilitates direct single-cell analysis of LAB, including morphology.
  • The method is a valuable tool for investigating LAB enzyme activity and protein expression.