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Salmonella typhimurium metE operator-constitutive mutations.

L S Plamann1, M L Urbanowski, G V Stauffer

  • 1Department of Microbiology, University of Iowa, Iowa City 52242.

Gene
|December 15, 1988
PubMed
Summary

Mutations in Salmonella typhimurium

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Area of Science:

  • Microbiology and Molecular Genetics
  • Bacterial Gene Regulation

Background:

  • The metE gene in Salmonella typhimurium is crucial for methionine biosynthesis.
  • Its expression is regulated by the metJ repressor and vitamin B12.

Purpose of the Study:

  • To identify and characterize operator-constitutive mutations in the metE control region.
  • To investigate the interaction between the metJ repressor and vitamin B12-mediated repression.

Main Methods:

  • Utilized a metE-lacZ fusion phage (lambda Elac) for mutant selection.
  • Screened for operator-constitutive mutations within the metE regulatory region.
  • Analyzed beta-galactosidase levels in lysogens under different conditions.

Main Results:

  • Identified mutations clustered in a tandemly-repeating palindrome region (5'-AGACGTCT-3'), the proposed metJ repressor binding site.
  • Mutant lysogens showed high beta-galactosidase levels, only partially repressed by methionine.
  • Vitamin B12 repression was disrupted in the absence of an active MetJ repressor.

Conclusions:

  • The metJ repressor binding site is critical for operator-constitutive mutations.
  • An interaction exists between the metJ repressor and the vitamin B12 repression system.
  • This interaction is likely mediated by the metH gene product.

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