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Updated: Jan 19, 2026

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Diagnostic Optical Sequencing.

Lee E Korshoj, Prashant Nagpal

    ACS Applied Materials & Interfaces
    |September 10, 2019
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces optical sequencing using Raman spectroscopy for accurate DNA and RNA analysis. This rapid, cost-effective diagnostic method shows promise for broad-spectrum biomarker detection in precision medicine.

    Keywords:
    Raman spectroscopybiomarker detectionhigh-throughput diagnosticsmultidrug-resistant bacteriaoptical sequencing

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    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Spectroscopy

    Background:

    • Precision medicine demands advanced diagnostic tools for multiomics analysis.
    • Current optical techniques face limitations in achieving single-nucleotide resolution.
    • Raman spectroscopy offers a potential solution by analyzing nucleobase content.

    Purpose of the Study:

    • To develop and validate an optical sequencing method for high-throughput, point-of-care diagnostics.
    • To assess the accuracy of optical sequencing for predicting nucleobase content in DNA and RNA.
    • To demonstrate the feasibility of this platform for pathogen identification.

    Main Methods:

    • Utilized Raman spectroscopy on positively charged silver nanoparticles for optical sequencing.
    • Measured label-free DNA k-mer blocks (k=10) and RNA.
    • Employed a content-scoring database searching algorithm for gene identification.

    Main Results:

    • Achieved 93.3% accuracy in predicting nucleobase content for DNA k-mers.
    • Demonstrated potential for transcriptomic and epigenetic studies with RNA and modified nucleobases.
    • Successfully identified a beta-lactamase gene and confirmed Pseudomonas aeruginosa pathogen using minimal data.

    Conclusions:

    • Optical sequencing using Raman spectroscopy is a feasible diagnostic platform.
    • The method offers high accuracy, cost-effectiveness, and potential for broad-spectrum biomarker detection.
    • Plasmonic substrates enable versatile data acquisition for rapid diagnostics.