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Related Experiment Videos

A bacterial pathogenicity determinant associated with necrotizing enterocolitis.

C A Carbonaro1, D A Clark, D Elseviers

  • 1Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.

Microbial Pathogenesis
|December 1, 1988
PubMed
Summary
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Infants with necrotizing enterocolitis (NEC) may be affected by bacteria with enhanced lactose fermentation. This ability, linked to Klebsiella pneumoniae, appears to be a key bacterial trait in NEC pathogenesis.

Area of Science:

  • Microbiology
  • Neonatal Research
  • Gastroenterology

Background:

  • Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease in premature infants.
  • The role of specific bacterial traits in NEC pathogenesis remains under investigation.
  • Enterobacteria are frequently implicated in NEC, but their specific pathogenic mechanisms require further elucidation.

Purpose of the Study:

  • To investigate the potential role of enhanced lactose fermentation in bacterial pathogenicity in necrotizing enterocolitis (NEC).
  • To identify bacterial genes associated with lactose fermentation and their contribution to NEC.

Main Methods:

  • Isolation and characterization of predominant enterobacteria from infants with NEC.
  • Induction of lactose operon expression in Klebsiella pneumoniae.

Related Experiment Videos

  • Pathogenicity testing using a rabbit ileal loop model for NEC.
  • Gene cloning and plasmid-mediated transfer to assess phenotype.
  • Construction and testing of a K. pneumoniae mutant deficient in lactose repressor synthesis.

    • Main Results:

    • A Klebsiella pneumoniae isolate from NEC infants exhibited partial lactose operon induction and pathogenicity in a rabbit model.
    • A segregant lacking this induction was non-pathogenic, and the responsible gene was cloned.
    • Introduction of the cloned gene restored both lactose fermentation and pathogenicity.
    • A K. pneumoniae mutant lacking lactose repressor was also pathogenic.

    Conclusions:

    • Enhanced lactose fermentation ability in enterobacteria may represent a significant bacterial pathogenic trait contributing to necrotizing enterocolitis (NEC).
    • The lactose repressor system in K. pneumoniae is implicated in its pathogenicity in the context of NEC.
    • Further research into bacterial metabolic capabilities is crucial for understanding and preventing NEC.