Omics Application of Bio-Hydrogen Production Through Green Alga Chlamydomonas reinhardtii

This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in Chlamydomonas reinhardtii. In this paper, critical genes (HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1) involved in H₂ metabolism were identified and analyzed for their function in H₂ accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H₂ production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H₂ metabolism. This review provides fundamental theory and an experimental basis for H₂ production, and further research effort is needed in this field.