Related Experiment Video

Updated: Jan 19, 2026

Quantification of Bacterial Alginate Using the Uronic Acid Carbazole Assay
04:17

Quantification of Bacterial Alginate Using the Uronic Acid Carbazole Assay

Published on: September 26, 2025

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Recent advances in the synthesis of carbazoles from indoles

Trapti Aggarwal1, Sushmita1, Akhilesh K Verma1

  • 1Synthetic Organic Chemistry Laboratory, Department of Chemistry, University of Delhi, Delhi-110007, India. averma@acbr.du.ac.in.

Organic & Biomolecular Chemistry
|September 10, 2019
PubMed

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Source: Al Ahmar, R., et al. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (2020)This video demonstrates a colorimetric assay to quantify alginate produced by Pseudomonas aeruginosa, using carbazole to detect uronic acids after acid hydrolysis. The method enables comparative analysis of alginate production normalized to bacterial biomass in research and clinical...
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