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An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

The protocol illustrates the use of histopathologic examination and immunohistochemistry to profile the folate receptor beta macrophage and its relationship with the total immune cell infiltrate in temporal artery biopsies in giant cell...

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other...

Single-Cell Culture of Bacteria Within Giant Vesicles

Single-Cell Culture of Bacteria Within Giant Vesicles

Source: Morita, M., et al. Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles. J. Vis. Exp. (2019).This video demonstrates a method for culturing and observing individual bacterial cells encapsulated inside giant vesicles anchored to a supported lipid bilayer. The system enables stable, long-term imaging of single-cell growth dynamics in a confined, physiologically relevant...

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

This article presents a method for preparing compound Giant Unilamellar Vesicles (cGUVs) with a vesicle-in-vesicle structure, with customized electrical conductivity in inner, annular, and outer regions. Electroformation synthesizes simple GUVs, which are transformed into stomatocytes and cGUVs via osmotic shock, providing a valuable model for studying the biophysics of nucleated...

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived...

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber...

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Related Experiment Video

Updated: Jan 19, 2026

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

Published on: February 8, 2019

Diplopia and Giant Cell Arteritis

Edsel B Ing¹, Neil R Miller, Martin Ten Hove

¹Department of Ophthalmology, University of Toronto, Toronto, Canada Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland Department of Ophthalmology, Queens University, Kingston, Canada Department of Ophthalmology, Beth Israel Deaconess Hospital, Boston, Massachusetts.

Journal of Neuro-Ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society

|September 10, 2019

Summary

No abstract available in PubMed .

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An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

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