Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Injecting Gryllus bimaculatus Eggs08:49

Injecting Gryllus bimaculatus Eggs

18.5K
Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic...
18.5K
Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology08:30

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

3.8K
This paper outlines basic methods to standardize important factors such as density, feed availability, hydration source, and environmental controls for the long-term rearing of laboratory cultures of the edible cricket, Gryllus...
3.8K
Obtaining Eggs from Xenopus laevis Females04:47

Obtaining Eggs from Xenopus laevis Females

15.1K
The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged...
15.1K
Probing the Limits of Egg Recognition Using Egg Rejection Experiments Along Phenotypic Gradients07:34

Probing the Limits of Egg Recognition Using Egg Rejection Experiments Along Phenotypic Gradients

8.6K
This protocol provides guidelines for running egg rejection experiments: outlining techniques for painting experimental egg models to emulate the colors of natural bird eggs, conducting fieldwork, and analyzing the collected data. This protocol provides a uniform method for conducting comparable egg rejection...
8.6K
Drosophila Egg Collection and Dechorionation: A Method to Remove the Outermost Egg Layer03:26

Drosophila Egg Collection and Dechorionation: A Method to Remove the Outermost Egg Layer

8.8K
The Drosophila embryo is surrounded by protective outer membranes. To gain access to the embryo, the chorion–the outermost of the membranes–is often removed. This video describes a commonly used method of dechorionation using sodium hypochlorite. The example protocol demonstrates the procedure while employing sterile techniques necessary for axenic or gnotobiotic animal...
8.8K
Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans02:25

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

6.5K
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies.  This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heterotypic intercellular adhesion tunes efficiency of cell-on-cell migration.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Integration of a neuronal RNAseq dataset with the draft Gryllus bimaculatus transcriptome refines gene predictions and highlights potential systematic response to injury.

PloS one·2026
Same author

The dynamic evolution of panarthropod germ cell specification mechanisms.

Development (Cambridge, England)·2026
Same author

A Simple Method for Estimating the Spatiotemporal Distribution of Phenoloxidase Proteins in Insect Tissues.

Bio-protocol·2026
Same author

Evidence for Non-optimal Codon Choice in Highly Transcribed Sex-Biased Genes of Drosophila melanogaster.

Genome biology and evolution·2026
Same author

HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.

Communications biology·2026
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Injecting Gryllus bimaculatus Eggs
08:49

Injecting Gryllus bimaculatus Eggs

Published on: August 22, 2019

18.5K

Injecting Gryllus bimaculatus Eggs.

Samantha K Barry1, Taro Nakamura2, Yuji Matsuoka3

  • 1Colby College.

Journal of Visualized Experiments : Jove
|September 10, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a new method for injecting cricket eggs to study gene function in developing organisms. This technique allows for various genetic manipulations, expanding research possibilities in non-model species.

More Related Videos

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology
08:30

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

Published on: June 8, 2022

3.8K
Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

15.1K

Related Experiment Videos

Last Updated: Jan 19, 2026

Injecting Gryllus bimaculatus Eggs
08:49

Injecting Gryllus bimaculatus Eggs

Published on: August 22, 2019

18.5K
Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology
08:30

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

Published on: June 8, 2022

3.8K
Obtaining Eggs from Xenopus laevis Females
04:47

Obtaining Eggs from Xenopus laevis Females

Published on: August 20, 2008

15.1K

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Genetic manipulation is crucial for understanding gene function in developing organisms.
  • Existing genetic tools are limited to traditional model systems, hindering research in diverse species.
  • Comparative and evolutionary studies require gene manipulation techniques adaptable to various organisms.

Purpose of the Study:

  • To describe a protocol for injecting cricket eggs to assay gene function during embryonic and larval development.
  • To provide a flexible and adaptable method for genetic manipulation in insects.
  • To enable functional genomics studies in non-model insect species.

Main Methods:

  • Collection and injection of cricket eggs using beveled needles.
  • Application of the technique for various experimental approaches, including reagent delivery.
  • Optimization of injection survival rates through practice.

Main Results:

  • A straightforward and flexible protocol for cricket egg injection was established.
  • Survival rates for buffer-only injections can reach up to 80% with practice.
  • The method supports diverse experimental manipulations, including RNA interference and CRISPR/Cas9 gene editing.

Conclusions:

  • This cricket egg injection technique offers a valuable tool for studying gene function in a non-model organism.
  • The method is adaptable to other insect species, broadening its applicability in evolutionary and developmental biology.
  • It facilitates a range of genetic and molecular experiments, advancing functional genomics research.