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Updated: Jan 19, 2026

Amphotericin-B Mediated Perforated Patch-Clamp Analysis: An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells
Published on: April 30, 2023
Nectriatide, a Potentiator of Amphotericin B Activity from Nectriaceae sp. BF-0114
Takashi Fukuda1,2, Kenichiro Nagai1, Akiho Yagi3
1Microbial Chemistry and Medicinal Research Laboratories, Graduate School of Pharmaceutical Sciences , Kitasato University , 5-9-1 Shirokane , Minato-ku, Tokyo 108-8641 , Japan.
Abstract:
A new compound, designated nectriatide (1), was isolated as a potentiator of amphotericin B (AmB) activity against Candida albicans from the culture broth of Nectriaceae sp. BF-0114. This structure was elucidated based on spectroscopic analyses (1D and 2D NMR data), chemical methods, and total synthesis. Compound 1 was a unique cyclotetrapeptide consisting of l-N-methyltyrosine, anthranilic acid, l-alanine, and l-valine. Compound 1 and several synthetic derivatives, including linear peptides, potentiated AmB activity against C. albicans by up to 16-fold (the MIC value of AmB decreased from 0.5 μg/mL to 0.031 μg/mL in combination with test compound).
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