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Related Concept Videos

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems06:18

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In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput...
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Genome editing tools such as the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system have greatly improved gene targeting efficiency in human induced pluripotent stem cells (hiPSCs). This manuscript describes a protocol for generating lineage specific hiPSC reporter using CRISPR/Cas system assisted homologous...
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Krishna Juluru1, Marta E Heilbrun1, Marc D Kohli1

  • 1From the Department of Radiology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, Box 29, New York, NY 10065 (K.J.); Department of Radiology, Emory University School of Medicine, Atlanta, Ga (M.E. H.); and Department of Radiology, University of California-San Francisco, San Francisco, Calif (M.D.K.).

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