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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice07:54

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.
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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal...
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An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions04:09

An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions

In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue...
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Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation08:34

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation

Here, we present a protocol to establish the antibiotic-induced pseudo-germ-free dextran sulfate sodium-induced colitis mouse model to investigate the role of gut microbiota in regulating the positive effects of Bacillus cereus on colitis.
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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization08:30

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization...
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An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions05:27

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

This article presents a unique method for analyzing host-microbiome interactions using a novel gut organ culture system for ex vivo experiments.
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