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Updated: Jan 19, 2026

A Peg Plate Biofilm Assay for Screening Antibacterial Agents
Preparation, characterization, and antibacterial properties of biofilms comprising chitosan and ε-polylysine
Zuolong Yu1, Guiwei Rao1, Yunxiao Wei1
1Biology and Environmental Engineering College, Zhejiang Shuren University, Hangzhou 310015, China.
Abstract:
Of late, the demand for food-packaging materials, in particular, multifunction packaging materials that are biodegradable; antibacterial; have good mechanical and barrier properties; and are edible and transparent, has increased considerably. In this study, we prepared chitosan (CS)/ε-polylysine (PL) biofilms with different CS-to-PL ratios. We studied the preparation, mechanical properties, microstructures, thermal stability, transparency, water-vapor permeability, oil permeability, and antibacterial properties of the composite CS/PL biofilms. The results demonstrate that CS/PL biofilms are mechanically strong, have good thermal stability, high transparency, low water-vapor and oil permeability, and extensive antibacterial properties that act against Escherichia coli, Bacillus subtilis, and yeast. Therefore, we therefore conclude that CS/PL biofilms are promising food-packaging materials.
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