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Updated: Jan 19, 2026
Cooperative Binding of Transcription Regulators
Simulation of live-cell imaging system reveals hidden uncertainties in cooperative binding measurements
Masaki Watabe1, Satya N V Arjunan1,2, Wei Xiang Chew1,3
1Laboratory for Biologically Inspired Computing, RIKEN Center for Biosystems Dynamics Research, Suita, Osaka 565-0874, Japan.
Abstract:
We propose a computational method to quantitatively evaluate the systematic uncertainties that arise from undetectable sources in biological measurements using live-cell imaging techniques. We then demonstrate this method in measuring the biological cooperativity of molecular binding networks, in particular, ligand molecules binding to cell-surface receptor proteins. Our results show how the nonstatistical uncertainties lead to invalid identifications of the measured cooperativity. Through this computational scheme, the biological interpretation can be more objectively evaluated and understood under a specific experimental configuration of interest.
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