Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

11.0K
We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in...
11.0K
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

13.7K
The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's...
13.7K
Mast Cell Degranulation Assay to Study the Effect of a Target Chemical04:08

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

1.4K
This video demonstrates a colorimetric assay to determine the degranulation activity of mast cells. The enzyme released by the mast cells hydrolyzes the substrate and produces a colored product. The degranulation activity of the mast cells was measured as the percentage release of enzymes outside the cell normalized with the total enzyme content in the...
1.4K
Assessment of Perigenital Sensitivity and Prostatic Mast Cell Activation in a Mouse Model of Neonatal Maternal Separation09:49

Assessment of Perigenital Sensitivity and Prostatic Mast Cell Activation in a Mouse Model of Neonatal Maternal Separation

9.7K
We are measuring perigenital mechanical sensitivity and mast cell activation in the prostate of male C57BL/6 mice that underwent an early life stress paradigm – neonatal maternal separation, in order to induce a preclinical model of chronic prostatitis/chronic pelvic pain...
9.7K
A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity04:17

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity

1.3K
This video demonstrates a peritoneal lavage method for isolating mature connective tissue-type mast cells from the peritoneal cavity of adult mice. Upon isolating a mixture of primary immune cells from the peritoneal cavity, selective proliferation of mast cells is performed under appropriate culture...
1.3K
An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells03:34

An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells

558
This video demonstrates a technique to visualize exocytosis in mast cells. The cells are incubated with FITC-dextran — a fluorescent reporter — which is taken up by cells inside secretory granules (SGs), where the acidic pH quenches its fluorescence. Upon stimulation of granule exocytosis, the medium's higher pH induces reporter fluorescence inside the fused SGs, which is visualized using a fluorescence...
558

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple sclerosis patients under treatment with interferon β1-a or ocrelizumab exhibit different T and B cell responses to SARS-CoV-2 vaccine.

Frontiers in immunology·2026
Same author

Allergen Immunotherapy and Possible Clinical Remission: Toward a Disease-Modifying Paradigm in Allergic Disorders.

Biomedicines·2026
Same author

Risk of intestinal involvement in mucocutaneous-onset Behçet's disease: data from the AIDA network registry.

Frontiers in immunology·2026
Same author

Vascular remodeling in asthma: from mechanisms to precision medicine.

Current opinion in allergy and clinical immunology·2026
Same author

A multispecialty consensus-based red flag checklist for the early recognition of ANCA-associated vasculitis.

Frontiers in immunology·2026
Same author

Clinical remission in allergy and clinical immunology practice: State of the art and World Allergy Organization (WAO) call to action.

The World Allergy Organization journal·2026
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

11.0K

Future Needs in Mast Cell Biology.

Gilda Varricchi1,2,3, Amato de Paulis4,5,6, Gianni Marone7,8,9,10

  • 1Department of Translational Medical Sciences (DISMET), University of Naples Federico II, 80138 Naples, Italy. gildanet@gmail.com.

International Journal of Molecular Sciences
|September 11, 2019
PubMed
Summary
This summary is machine-generated.

Mast cells, crucial immune cells, have diverse roles in health and disease. Research suggests distinct mast cell subsets (MC1 and MC2) may have opposing effects in cancer and heart conditions.

Keywords:
allergyatherosclerosiscancercancer-related inflammationmast cellmyocardial infarctionpredictive biomarkertumor-associated mast cells

More Related Videos

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

13.7K
Mast Cell Degranulation Assay to Study the Effect of a Target Chemical
04:08

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

1.4K

Related Experiment Videos

Last Updated: Jan 19, 2026

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

11.0K
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

13.7K
Mast Cell Degranulation Assay to Study the Effect of a Target Chemical
04:08

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

1.4K

Area of Science:

  • Immunology
  • Cell Biology
  • Pathophysiology

Background:

  • Mast cells, first described in 1878, have complex and not fully understood pathophysiological roles.
  • Mast cell heterogeneity is increasingly recognized, with subtypes identified through transcriptomic analysis of distinct anatomical locations.
  • These immune cells can influence homeostasis, potentially contributing to its restoration or disruption.

Purpose of the Study:

  • To explore the multifaceted roles of mast cells in various pathophysiological processes.
  • To investigate the potential for distinct mast cell subsets to exert opposing effects in disease.
  • To highlight the strategic locations of mast cells in the heart and their involvement in cardiac conditions.

Main Methods:

  • Review of existing literature on mast cell subpopulations and transcriptomic analysis.
  • Examination of mast cell involvement in tumorigenesis (solid and hematologic tumors).
  • Analysis of mast cell presence and function in cardiac tissues (myocardium, atherosclerotic plaques, aortic valve).

Main Results:

  • Mast cells accumulate in tumors, suggesting roles in both promoting and limiting tumorigenesis.
  • Hypothesized existence of at least two major mast cell subsets: MC1 (anti-tumorigenic) and MC2 (pro-tumorigenic).
  • Cardiac mast cells are implicated in both physiological and pathological cardiac processes, potentially with distinct functional effects.

Conclusions:

  • Mast cells exhibit functional diversity, with different subsets or mediators potentially mediating opposing roles in disease.
  • Distinct mast cell subsets, similar to cardiac macrophages, may exert varied effects in cardiac pathophysiology.
  • Further research is needed to fully elucidate the diverse functions of mast cells in health and disease.