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Related Concept Videos

Chemical Dimerization-Induced Protein Condensates on Telomeres08:52

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This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin.  The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and fluorescence in situ hybridization...
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Creating chemically induced protein dimerization systems with desired affinity and specificity for any given small molecule ligand would have many biological sensing and actuation applications. Here, we describe an efficient, generalizable method for de novo engineering of chemically induced dimerization systems via the stepwise selection of a phage-displayed combinatorial single-domain antibody...
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We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy...
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Multi-input and Multi-variable systems01:22

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Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
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Light-Controlled Fermentations for Microbial Chemical and Protein Production08:37

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Optogenetic control of microbial metabolism offers flexible dynamic control over fermentation processes. The protocol here shows how to set up blue light-regulated fermentations for chemical and protein production at different volumetric...
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The cell permeable crosslinker DSP [dithiobis-(succinimidyl propionate)] stabilizes transient and labile interactions in vivo, which allows their isolation using stringent protein complex purification techniques. Here we present a technique for crosslinking cells grown in culture followed by isolation of protein complexes by...
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Related Experiment Video

Updated: Jan 19, 2026

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Multi-input chemical control of protein dimerization for programming graded cellular responses.

Glenna Wink Foight1,2, Zhizhi Wang2,3, Cindy T Wei1

  • 1Department of Chemistry, University of Washington, Seattle, WA, USA.

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Researchers developed a novel system for precise, multi-drug control of cellular functions. This versatile tool enables complex programming of cellular processes and engineering of cell therapies.

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Area of Science:

  • Cell Biology
  • Synthetic Biology
  • Biochemistry

Background:

  • Existing chemical and optogenetic methods offer limited single-input, single-output control over protein function.
  • There is a need for more sophisticated systems to program diverse post-translational cellular behaviors.

Purpose of the Study:

  • To develop a versatile system for multi-input control of protein function using a single receiver protein.
  • To enable diverse and programmable post-translational cellular responses.

Main Methods:

  • Engineered a single protein receiver capable of integrating multiple drug inputs.
  • Utilized a suite of engineered reader proteins to translate drug inputs into variable dimerization states.
  • Demonstrated control over transcription and mammalian cell signaling pathways.

Main Results:

  • Successfully programmed graded and proportional dual-output control of cellular functions.
  • Applied the system to titrate competing Rac and Rho GTPase activities, controlling cell morphology.
  • Showcased the system's versatility in programming diverse cellular responses.

Conclusions:

  • The developed system provides a powerful new tool for post-translational control of cellular processes.
  • This platform enables sophisticated engineering of mammalian cellular functions and cell therapies.