Related Concept Videos

Ex Situ Normothermic Machine Perfusion of Donor Livers12:13

Ex Situ Normothermic Machine Perfusion of Donor Livers

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver...
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Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

In this protocol, a model of porcine orthotopic liver transplantation after static cold storage of donor organs for 20 h without the use of a veno-venous bypass during engraftment is described. The approach uses a simplified surgical technique with minimization of the anhepatic phase and sophisticated volume and vasopressor...
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Procurement of Parathyroid Glands from Living Donor Pigs and Ex Vivo Identification08:07

Procurement of Parathyroid Glands from Living Donor Pigs and Ex Vivo Identification

This protocol presents a rapid and non-lethal method for procuring parathyroid glands from pigs. The procedure consists of three main steps: porcine preparation with anesthesia, unilateral thymus retrieval, and ex vivo identification and extraction of the intrathymic parathyroid gland.
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Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation05:25

Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation

This protocol outlines the methodology of establishing a porcine model utilizing variable temperature-controlled Machine Perfusion (MP) for the preservation of the donor liver, followed by orthotopic liver transplantation (OLTx). It aims to promote the success rate of OLTx using donor donation after circulatory death (DCD) liver and establish a stable...
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In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion12:35

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and...
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Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels08:37

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Here, we present a protocol to establish a long-term liver cold storage and transplantation model in Daurian ground squirrel (DGS), which has been technically challenging in standard rodent models.
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