Creation of a Model of Co-Culturing of Sertoli-Like Mouse Cells with Spermatogonial Cells

Sertoli-like cells is a cell population in the testes of adult mice capable of growth in culture and expressing many genes typical of Sertoli cells and supporting the development of germ cells in the gonad. A technique of co-culturing of Sertoli-like cells with spermatogonial cells was proposed that allows maintaining the growth and viability of germ cells and inducing their differentiation. This technique can provide the basis for obtaining fully differentiated germ cells in culture through using Sertoli-like cells as the supporting somatic cells.