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Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Histone Modification

Histone Modification

Detection of Histone Modifications in Plant Leaves

Detection of Histone Modifications in Plant Leaves

A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological...

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.

Detecting Histone Modifications in Yeast Cells with Neurodegenerative Protein Overexpression

Detecting Histone Modifications in Yeast Cells with Neurodegenerative Protein Overexpression

Source: Bennett, S. A., et al., Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J. Vis. Exp. (2019).This video demonstrates the procedure of assessing histone modifications in yeast cells overexpressing neurodegenerative proteins. The steps include cell lysis, centrifugation, electrophoresis, membrane transfer, and antibody-based detection of modified...

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

This protocol outlines a fully integrated workflow for characterizing histone post-translational modifications using mass spectrometry (MS). The workflow includes histone purification from cell cultures or tissues, histone derivatization and digestion, MS analysis using nano-flow liquid chromatography and instructions for data analysis. The protocol is designed for completion within 2 - 3...

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Related Experiment Video

Updated: Jan 19, 2026

Histone Modification: Acetylation and Methylation

Histone Modification: Acetylation and Methylation

Histone Modifications as Biomarkers for Immunotherapy.

Erin M Taylor¹, Brian Koss¹, Lauren E Davis¹

¹Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 11, 2019

Summary

Histone posttranslational modifications (PTMs) show promise as biomarkers for predicting cancer immunotherapy response. Assaying these epigenetic markers can guide treatment strategies and identify new therapeutic targets.

Keywords:

Biomarker Epigenetics Histone Immunotherapy Posttranslational modification

More Related Videos

Histone Modification

Histone Modification

Detection of Histone Modifications in Plant Leaves

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

Related Experiment Videos

Last Updated: Jan 19, 2026

Histone Modification: Acetylation and Methylation

Histone Modification: Acetylation and Methylation

Histone Modification

Histone Modification

Detection of Histone Modifications in Plant Leaves

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

Area of Science:

Oncology
Immunology
Epigenetics

Background:

Immunotherapy has transformed cancer treatment, but patient responses vary significantly.
Identifying biomarkers for immunotherapy responsiveness is crucial for personalized medicine.
Epigenetic modifications, specifically histone posttranslational modifications (PTMs), are implicated in cancer and immune cell function.

Purpose of the Study:

To explore the potential of histone PTMs as biomarkers for predicting immunotherapy response.
To identify molecular pathways involved in immunotherapy resistance.
To provide a methodological framework for assaying histone PTMs.

Main Methods:

Review of recent advancements in immunotherapy and biomarker discovery.
Analysis of histone PTMs in cancer and immune cells.
Development of a workflow for high-throughput assaying of histone PTMs.

Main Results:

Histone PTMs are promising candidates for biomarkers due to their abundance and assay accessibility.
Dysregulated histone PTMs are observed in both cancer and immune cells.
A methodological workflow for histone PTM assaying is proposed.

Conclusions:

Histone PTMs can serve as valuable biomarkers for predicting patient response to cancer immunotherapy.
Targeting dysregulated epigenetic pathways may overcome immunotherapy resistance.
High-throughput assaying of histone PTMs offers a feasible approach for clinical application.