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Injecting Recombinant Adeno-Associated Vectors in a Spinal and Bulbar Muscular Atrophy Mouse Model02:44

Injecting Recombinant Adeno-Associated Vectors in a Spinal and Bulbar Muscular Atrophy Mouse Model

Source:Pourshafie, N., et al. Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents. J. Vis. Exp. (2018).In this video, a spinal and bulbar muscular atrophy or SBMA-affected mouse undergoes tail vein injection of recombinant AAV (Adeno-associated vectors) vectors carrying a plasmid encoding therapeutic microRNA. The vector produces microRNA that binds to mutant AR (Androgen receptor)-mRNA (messenger ribonucleic acid),...
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Detection of Brain Atrophy by Hematoxylin and Eosin Staining01:21

Detection of Brain Atrophy by Hematoxylin and Eosin Staining

To detect brain atrophy, begin with slides carrying the deparaffinized brain sections from neurodegenerative mouse models at various...
194
Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice10:50

Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice

The tibial nerve transection model is a well-tolerated, validated, and reproducible model of skeletal muscle atrophy. The model surgical protocol is described and demonstrated in C57Black6 mice.
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Culturing Microglia from the Neonatal and Adult Central Nervous System11:28

Culturing Microglia from the Neonatal and Adult Central Nervous System

We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial...
28.8K
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE20:51

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.
16.7K
Intrathecal Delivery of a Test Compound to the Rat Central Nervous System05:04

Intrathecal Delivery of a Test Compound to the Rat Central Nervous System

Source: Chen, Y., et al. Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System. J. Vis. Exp. (2019)This video demonstrates the delivery of a test compound into the cerebrospinal fluid (CSF) through a catheter implanted in the lumbar spinal column of a rat model. The compound circulates along the CSF to the brain and spinal cord, enters neurons, and offers therapeutic...
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