Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity

Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization. The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively. The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure. Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass. Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency). The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line. Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.