Related Concept Videos

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development09:23

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development

The zebra finch (Taeniopygiaguttata) is a valuable model organism; however, early stages of zebra finch development have not been extensively studied. The protocol describes how to dissect early embryos for developmental and molecular applications.
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Operant Conditioning Task to Measure Song Preference in Zebra Finches06:40

Operant Conditioning Task to Measure Song Preference in Zebra Finches

We describe a technique to evaluate song preference in zebra finches. Females are placed in a two-chambered cage and song preference is measured by the number of times she triggers the playback of one song by landing on a perch within one chamber, compared with triggering a different song in the second chamber. Perch landings are counted using infrared...
6.9K
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic...
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Zebra II as A Novel System to Record Electrophysiological Signals in Zebrafish06:15

Zebra II as A Novel System to Record Electrophysiological Signals in Zebrafish

The study introduces Zebra II, an advanced system for prolonged ECG acquisition and analysis in zebrafish. This system features an independent perfusion system and multiple-point electrodes for handling multiple fish in controlled environments. Acute effects of Amiodarone were assessed and analyzed with wild-type zebrafish.
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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum08:26

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of...
38.9K
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish13:25

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL,...
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