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Updated: Jan 19, 2026
Operation of High-Performance Liquid Chromatography HPLC
Published on: April 30, 2023
Determination of doravirine in human plasma using liquid-liquid extraction and HPLC-MS/MS
Rajesh Desai1, Brad Roadcap1, Dina Goykhman1
1Department of Pharmacokinetics, Pharmacodynamics & Drug Metabolism, MRL, Merck & Co., Inc., West Point, PA 19486-0004, USA.
Abstract:
Aim: A method to quantitate doravirine (MK-1439) in human plasma has been developed to support human clinical trials designed to evaluate the safety, pharmacokinetics and efficacy of the compound. Methodology & results: The analyte was extracted using liquid-liquid extraction, separated on a reverse phase HPLC column, and detected on an API-4000 mass spectrometer using a Turbo-Ion spray source in positive ionization mode coupled with multiple reaction monitoring mode was used for quantification. The dynamic range for the assay was 0.02-10 ng/ml using 100 μl of human plasma. Conclusion: The assay was found to be sensitive, selective and reproducible and applied to support the doravirine clinical development program.
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